Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. His-tag mouse mAb recognizes His-tag placed at N-terminal, C-terminal, and internal regions of fusion proteins.
Product Name:His-tag mouse monoclonal antibody
Clone Number: 5B8
Isotype: IgG1
Immunogen: A synthetic peptide HHHHHH coupled to KLH.
Tested applications: WB. Not yet tested in other applications.
Recommended dilutions: WB: 1:5000
Optimal dilutions/concentrations should be determined by the end user.
Form:Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: Hisantibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.