Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase is routinely used for digestion. Zymo Research’s liquid and lyophilized forms of the yeast lytic enzyme Zymolyase is prepared from Arthrobacter luteus. The buffer used to solubilize the Zymolyase is optimized to confer maximal levels of enzymatic activity. The main enzymatic activities are ß-1,3-glucanase and ß-1,3-glucan laminaripentaohydrolase, which hydrolyze glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.