roduct List

 pAX01, a 7781 bp plasmid in E. coli JM109; integrates into the B. subtilis chromosome at lacA with, allows for xylose-inducible expression of cloned insert.

Recovery

1.Obtain an LB agar plate with the appropriate antibiotic.

2.Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A

sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3.Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4.Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second

section of the plate, to create streak #2.

5.Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak

#3.

6.Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

KEYWORDS    pAX01

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7781)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported 2015-4-18 from MLCC

            http://www.miaolingbio.com

FEATURES             Location/Qualifiers

     source          1..7781

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     terminator      660..687

                     /note="rrnB T2 terminator"

                     /note="transcription terminator T2 from the E. coli rrnB 

                     gene"

     terminator      706..792

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 terminator"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     promoter        2078..2182