ZR Tissue & Insect DNA MidiPrep™
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Simple method for the isolation of DNA from fresh, frozen or stored insect and arthropod specimens in as little as 25 minutes. |
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The organic denaturant/proteinase K-free procedure is ideal for processing small organisms including mosquitoes, bees, lice, ticks, and D. melanogaster. |
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State-of-the-art, ultra-high density BashingBeads™ are fracture resistant, chemically inert and can be used with any bead mill, disrupter, or vortex that can accommodate standard 50 ml tubes. |
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Compatible with mammalian tissues, cultured cells, and whole blood.
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SPECIFICATIONS
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Formats: Bead Beating, Spin/Vacuum Filtration, and Spin Column Purification. |
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Sample Sources: Samples (n ≥ 2.5 and ≤ 125 mg) of fresh, frozen, or stored insects including: mosquitoes, bees, lice, ticks, D. melanogaster, etc. Also, compatible with fresh or frozen mammalian tissues (e.g., soft tissues like liver and brain) as well as cultured cells, and whole blood. |
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DNA Yield: Expected yields can range from 1 - 5 µg DNA per mg specimen sampled. For mammalian tissues, yields are 1-3 µg DNA per mg of skeletal, heart and brain tissues and 3-5 µg DNA per mg of liver, kidney, and lung tissues. Whole blood will yield from 3-7 µg DNA per 100 µl. |
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DNA Purity: High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260 nm/280 nm > 1.8, A260 nm/230 nm > 2.0 |
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DNA Size Limits: Capable of recovering genomic DNA sized fragments from 100 bp to ≥ 40 kb. Typical fragment sizes range from 25 kb-35 kb. If present, parasitic and viral DNA will also be recovered. |
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DNA Recovery: Typically, up to~ 125 µg total DNA is eluted into ≥ 150 µl DNA Elution Buffer per sample. |
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Equipment: Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter. |
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DESCRIPTION
The ZR Tissue & Insect DNA MidiPrep™ is designed for the simple, rapid isolation of up to 125 µg total DNA (e.g., genomic, viral, mitochondrial) from small amounts of fresh, frozen or stored insect specimens including mosquitoes, bees, lice, ticks, and D. melanogaster. The procedure is easy and can be completed in as little as 20 minutes: samples are added directly to a ZR BashingBead™ Lysis/Filtration Tube and rapidly and efficiently lysed by bead beating without using organic denaturants or proteinases. The DNA is isolated and purified using our Fast Spin column technology and is ideal for downstream molecular-based applications including PCR, endonuclease digestion, array, genotyping, etc. PCR inhibitors are effectively removed during the purification process. Also, the procedure is compatible with mammalian tissues, whole blood, and cultured cells. A schematic of the ZR Tissue & Insect DNA MidiPrep™ procedure is shown below.
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ORDERING INFORMATION:
| Products |
Description |
Protocol
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Cat No.
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Size
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Price
(RMB)
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| ZR Tissue & Insect DNA MidiPrep™ |
Rapid isolation of up to 125 µg total DNA from small amounts of fresh, frozen or stored insect specimens |
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D6115
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25 preps.
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