• 靶标种属Chicken
• 经测试应用WB, IHC-Pmore details
• 免疫原

  Full length protein corresponding to Chicken IgY.

• 偶联物HRP

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.4
  Preservative: 0.1% Proclin
  Constituents: PBS, Sodium chloride, Sodium phosphate, 30% Glycerol, 1% BSA
•  
• 纯度Purified via His tag
• 纯化说明This product is a recombinant protein produced in E. coli.
• 克隆单克隆
• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

应用

Anti-Chicken IgY VHH Single Domain Antibody (HRP) 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chicken IgY sdAb (ab191865)

  IHC image of Anti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865) staining in formalin fixed paraffin embedded human lung adenocarcinoma tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab107115 (1.0µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865, 0.5µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Western blot - sdAb anti-Chicken IgY HRP (ab191865)
  All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
  
  Lane 1 : HeLa Whole Cell Lysate
  Lane 2 : Jurkat Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Anti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865) at 0.1 µg/ml
  developed using the ECL technique
  
  Performed under reducing conditions.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chicken IgY sdAb (ab191865)

  IHC image of Anti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865) staining in formalin fixed paraffin embedded human lung adenocarcinoma tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with chicken polyclonal antibody to BrdU (ab92837, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865, 0.125µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Western blot - sdAb anti-Chicken IgY HRP (ab191865)
  All lanes : Anti-GAPDH antibody (ab83956) at 1 µg/ml
  
  Lane 1 : HeLa Whole Cell Lysate
  Lane 2 : NIH 3T3 Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Anti-Chicken IgY VHH Single Domain Antibody (HRP) (ab191865) at 0.1 µg/ml
  developed using the ECL technique
  
  Performed under reducing conditions.