• 靶标种属Rabbit
• 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.
• 经测试应用ICC, IHC-P, ELISA, WBmore details
• 偶联物HRP

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C.
• 存储溶液Preservative: 0.1% Proclin
  Constituents: 0.2% BSA, PBS
• 浓度1 mg 浓度为 1 mg/ml 
  
• 纯度Immunogen affinity purified
• 纯化说明Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共轭说明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

  • Secondary antibodies
   
  • anti-Rabbit
   
  • IgG
   
  • Enzyme
   
  • HRP

应用

Goat Anti-Rabbit IgG Fc (HRP) 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

  IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is taken from an identical assay without primary antibody.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)
  Ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.