Product: Anti-Bfl-1 pAb
Cat. No.: PC-520 (100 μg)
Synonyms:
Anti-Bcl-2-Related Protein A1, Hemopoietic-
Specific Early Response Protein.
Background:
Apoptosis plays a major role in normal organism
development, tissue homeostasis, and removal
of damaged cells and is caused by caspase
activation. Proteins that comprise the Bcl-2
family appear to control the activation of these
enzymes. One such member is multi-domain
anti-apoptotic protein Bfl-1, which is overexpressed
in stomach and other cancers. Bfl-1
can interact with Bax and suppress apoptosis by
inhibiting the release of cytochrome c and
caspase-3 activation. It is upregulated in
cisplatin-resistant human bladder tumors,
suggesting that its expression may be important
for cisplatin resistance and inhibition of
apoptosis in cancer cells. At least two isoforms
of Bfl-1 are known to exist. Presumably due to
post-translational modifications, Bfl-1 is often
observed at higher molecular weight in SDSPAGE
than its predicted molecular weight would
suggest.
Specificity:
Recognizes Bfl-1.
Positive Control:
Mouse kidney lysate.
Species Reactivity:
Human, mouse and rat. Other species not
tested.
Host:
Rabbit
Immunogen:
A 14 amino acid peptide from near the aminoterminus
of human Bfl-1 (GenBank Accession
No. NP_004040).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Application and Suggested Dilution:

Western blot: Use at 1-2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Western blot analysis of Bfl-1 in mouse
kidney tissue lysate with anti-Bfl-1 pAb at (A) 1 μg/mL
and (B) 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product