Product: Anti-DAD1 pAb
Cat. No.: PC-464 (100 μg)
Synonyms:
Defender against cell death 1
Description:
Defender of cell death 1 (DAD1) was initially
discovered in BHK21 cells as a negative
regulator of programmed cell death, a process
important for normal organism development and
tissue homeostasis. DAD1 was later shown to
be a subunit of the mammalian oligosaccharyltransferase
complex and is required for its
function and structural integrity. Mice lacking
DAD1 express abnormal N-linked glycoproteins
and undergo increased apoptotic-associated
embryonic death. Furthermore, over-expression
of DAD1 mRNA is seen in some human hepatocellular
carcinomas, indicating it may also play a
role in carcinogenesis. It should be noted that
DAD1 is not related to the inhibitor of apoptosis
proteins (IAP) family and does not contain any
baculo-viral IAP repeat (BIR) domains.
Host:
Rabbit
Species Reactivity:
Human
Immunogen:
A peptide corresponding to 14 amino acids near
the C-terminus of human DAD1 (GenBank
accession no. AAH09798).
Format:
100 μg at 1 mg/mL of polyclonal IgG in PBS
containing 0.02% sodium azide. Ion-exchange
chromatography purified.
Storage:
Store at 4°C.
Applications:

Western blot: Use at 0.5 to 2 μg/mL. Despite
its predicted molecular weight, DAD1
migrates at 22 kDa in SDS-PAGE.

Immunohistochemistry: Use at about 10
μg/mL with HepG2 cells.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.