来源
用途
交叉反应性
分子量
Rabbit
WB, IP, IHC
H, M, R
38.0kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000-1:2,000
1:10-1:50
-
1:50-1:200
-
-
-
-
抗体详细信息如下:
About this Antibody
Name
HtrA2/Omi Rabbit Monoclonal Antibody
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype
IgG
Purification
Affinity purification
About the Immunogen
Immunogen
Recombinant protein
Gene ID
27429(Human); 64704(Mouse); 297376(Rat)
SwissProt
O43464(Human); Q9JIY5(Mouse); B0BNB9(Rat)
Synonyms
OMI; PARK13; PRSS25
Category
Apoptosis
Background
The human homolog of the E. Coli htrA gene product, HtrA, is identified in osteoarthritic cartilage and is repressed in SV40-transformed fibroblast. The gene encoding HtrA protein is highly conserved among mammalian species and belongs to the serine protease family. The HtrA protein contains an IGF-binding domain and exhibits endoproteolytic activity, including autocatalytic cleavage. HtrA is a secreted protein that is expressed in heterologous systems. HtrA plays a role in the degradation of denatured proteins and cell growth regulation. Human HtrA2 (also designated Omi), a novel member of the HtrA serine protease family, is highly homologous to HtrA (also known as L56 and HtrA1). HtrA2 is a ubiquitously expressed nuclear protease that is capable of autoproteolysis. The HtrA2 protein exists as two polypeptides and as an alternatively spliced form called D-Omi, which is predominately expressed in the kidney, colon and thyroid. Due to a modified PDZ domain, D-Omi does not interact with the known partner of HtrA2, the Mxi2 protein. Like HtrA, HtrA2 is involved in the degradation aberrantly folded proteins during conditions of cellular stress, suggesting that it may possess a chaperone-like role under normal conditions.
包装清单:
产品编号
产品名称
包装
AF1855
HtrA2/Omi Rabbit Monoclonal Antibody
50μl
AZ050
Western一抗稀释液
50ml
-
说明书
1份
保存条件:
HtrA2/Omi Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
代表性图片：
Fig. 1. Western blot analysis of HtrA2 on 293 cell lysates using anti-HtrA2 antibody at 1/1,000 dilution.
Fig. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.
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