分子量:24 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Biotinylated Human FcGR2A / CD32a (H131) by the LAL method.

Buffer:PBS, pH7.4

生物活性:The bioactivity is measured by its binding ability to Ipilimumab in a SPR assay.
Immobilized Human FcGR2A / CD32a (H131), His Tag (Cat# CDA-H82E6) can bind Ipilimumab with affinity constant of around 2.3 uM.


产品特性:This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 24 kDa. The predicted N-terminus is Ala 36. The reducing (R) protein migrates as 30-38 kDa in SDS-PAGE due to glycosylation.

产品背景:Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. 
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIMbearing receptors may also be coexpressed and coengaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.