生长抑制DNA损伤基因GADD45β抗体-分析方法-资讯-生物在线

生长抑制DNA损伤基因GADD45β抗体

作者:上海雅吉生物科技有限公司 2020-11-03T00:00 (访问量:614)

  

中文名称 生长抑制DNA损伤基因GADD45β抗体
别    名 GA45B_HUMAN; GADD45 Beta; Gadd45b; GADD45BETA; Growth arrest and DNA damage inducible 45 beta; Growth arrest and DNA damage inducible beta; Growth arrest and DNA damage-inducible protein GADD45 beta; Growth arrest and DNA-damage-inducible protein GADD45 beta; MYD118; Myeloid differentiation primary response gene; Myeloid differentiation primary response protein MyD118; Negative growth regulatory protein MyD118; Negative growth-regulatory protein MyD118.  
研究领域 肿瘤  细胞生物  免疫学  信号转导  细胞凋亡  生长因子和激素  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 18kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GADD45B.:81-160/160 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

Function:
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

Subunit:
Interacts with GADD45GIP1.

Similarity:
Belongs to the GADD45 family.

SWISS:
O75293

Gene ID:
4616

Database links:

Entrez Gene: 4616 Cow

Entrez Gene: 4616 Human

Entrez Gene: 17873 Mouse

Entrez Gene: 299626 Rat

Omim: 604948 Human

SwissProt: Q5E9A5 Cow

SwissProt: O75293 Human

SwissProt: P22339 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GADD45β; GADD45 β; GADD45-β.
产品图片 Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GADD45B) Polyclonal Antibody, Unconjugated (bs-5904R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GADD45B Polyclonal Antibody, Unconjugated(bs-5904R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GADD45B Polyclonal Antibody, Unconjugated(bs-5904R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-GADD45B antibody (bs-5904R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
上海雅吉生物科技有限公司 商家主页

地 址: 上海市闵行区元江路5500号第1幢5658室

联系人: 王源

电 话: 15301693058

传 真: 021-34661275

Email:yajikit@163.com

相关咨询

人原代附睾管上皮细胞 (2021-07-08T14:16 浏览数:7662)

雅吉生物热烈庆祝中国建党100周年 (2021-07-02T09:17 浏览数:3658)

15P-1 (小鼠睾丸上皮细胞) (STR鉴定正确) (2021-06-23T09:59 浏览数:6114)

客户细胞培养过程中常见的问题说明 (2021-06-18T09:54 浏览数:7976)

免疫荧光鉴定步骤 (2021-06-18T09:54 浏览数:9428)

人真皮成纤维细胞的分离培养制备方式 (2021-06-17T09:04 浏览数:6092)

原代角质形成细胞的分离培养方法 (2021-06-17T09:03 浏览数:9854)

钻石探头量子显微镜可以探索纳米微观世界的奥秘 (2021-06-16T09:24 浏览数:6281)

过氧化物酶体活化受体可以加剧肿瘤形成 (2021-06-16T09:23 浏览数:7006)

式细胞术数据分析之——圈门技巧 (2021-06-15T10:11 浏览数:7378)

ADVERTISEMENT